WebCycloheximide (Actidione) 0.5 g. Chloramphenicol 0.05 g. Yeast extract 5 g. Gentamicin (40 mg/ml) 0.56 mL. Distilled water 1000 mL. Method. Soak all ingredients, except Gentamicin, in 100 mL water. Boil remaining water, add to soaking ingredients, and bring … WebCycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, with IC50 of 532.5 nM.; IC50 value: 532.5 nM; Target: Antifungal; Cycloheximide is a highly effective antibiotic with activity against mold, yeast, and phytopathogenic fungi, with lower activity against bacteria. ... ACD/LogD (pH 7.4): 0.83 ACD/BCF (pH 7.4): 2.52 ...
NADcapPro and circNC: methods for accurate profiling of NAD …
WebTo measure protein half-lives in cultured mammalian cells (HEK293), we used cycloheximide at a concentration of 100 mkg/ml. The control protein which is GFP fused to the C-terminal degron of... WebProduct name : Cycloheximide Product Number : 01810 Brand : Sigma Index-No. : 613-140-00-8 CAS-No. : 66-81-9 1.2 Relevant identified uses of the substance or mixture and uses advised against ... pH No data available e) Melting point/freezing point Melting point/range: 110 - 118 °C (230 - 244 °F) f) Initial boiling point and boiling range famous music from romeo and juliet ballet
The mechanism of cycloheximide inhibition of protein synthesis …
WebApr 13, 2024 · Reactions were stopped with 100 μL of stop solution (50 mM Tris pH 8.0, 20 mM EDTA, and 0.1 µg/mL glycogen), and RNA was precipitated with 500 μL ethanol by incubating for 30 min at –80 °C ... WebCycloheximide ≥95% (HPLC) Synonym (s): 3- [2- (3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A Empirical Formula (Hill Notation): C15H23NO4 CAS Number: 66-81-9 Molecular Weight: 281.35 Beilstein: 88868 EC … Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life. Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation. famous music hall artists