WebConsiderations for Flow Cytometry Gating. There are a number of factors to consider and incorporate into your FACS staining and gating strategy, including: Compensating for spectral overlap Using appropriate gating … WebJun 2, 2024 · Allophycocyanin (APC) is a 105 kDa protein, with an extinction coefficient of 700,000 cm -1 M -1 and a quantum yield of 0.68. APC is maximally excited at 652 nm (with a secondary maximum at 625 nm) …
FITC anti-mouse IgG - BioLegend
WebThe two most commonly used fluorochromes in flow cytometry are fluorescein isothiocyanate (FITC) and phycoerythrin (PE). The two key properties of these dyes that make them preferred tags are that they are both excited with a 488 nm laser and that their emission spectra are distinct, with FITC at 530 nm (green) and PE at 570 – 575 nm … WebPrint this protocol. Fluorescence activated cell sorting (FACS) of live cells separates a population of cells into sub-populations based on fluorescent labeling. Sorting involves more complex mechanisms in the flow … does the mother or father carry the twin gene
FITC Mouse Anti-Human IgM - BD Biosciences
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