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Peasy-blunt e1

WebAug 26, 2024 · The PCR products were ligated into a pEASY-Blunt E1 Expression vector (TransGen Biotech, China) and transformed into Escherichia coli Trans-T1 ( Liu et al., 2024 ). After sequence confirmation by Sangon Biotechnology (Shanghai, China), the positive recombinant plasmids were designated as pEASY-Blunt E1-PiGSTd1. WebMay 1, 2024 · The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. Upon reaching an OD 600 of 0.6–0.8, expression in the …

苹果MdBT2在防治苹果花叶病中的应用

WebSDS-PAGE gel electrophoresis of BoaZDS expressed in E. coli M: Protein marker (6.5-200 kD); 1: Non-induced control; 2: Control induced for 8 h; 3,5,7: Non-induced expression products of pEASY... WebAug 28, 2024 · regulated by ABA treatment. Finally, the prokaryotic expression vector pEASY®-Blunt E1-IrUGT86A1 was successfully used to express about 53 KD of IrUGT86A1-like protein. This research builds a foundation for further investigation on the function of this gene in the synthesis and modification of secondary metabolites. glock difference in generations https://ambiasmarthome.com

SDS-PAGE gel electrophoresis of BoaZDS expressed in E

WebJul 18, 2024 · To express RdRp in E.coli, RdRp gene was inserted into pEASY-Blunt E1 Expression Vector (TransGen Biotech). And the recombinant p... View. Figure S5. Data. Feb 2024; Pramod Rathor; Tudor Borza; Web快速：利用DNA拓扑异构酶I，室温5分钟，快速克隆。 简单：酶已偶联到载体上，只需加入片段即可。 高效：载体（已偶联酶）和片段双分子碰撞，连接成功率高。 pEASY® … WebpEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. glock discontinued knives

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Peasy-blunt e1

Overexpression of the glucosyltransferase gene - ScienceDirect

WebFree Trial pEASY-Blunt E1 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Sequence Author: TransBionovo … WebOct 15, 2024 · UGT74B1 CDS was ligated with the pEASY-Blunt E1 expression vector and transformed into Trans 1-T1 (TransGene, China), yielding the recombinant vector pEASY -Blunt E1-BoaUGT74B1. The recombinant plasmid and empty plasmid were transferred into E. Coli Transetta (DE3) (TransGene, China).

Peasy-blunt e1

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WebFeb 28, 2024 · E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in normal liquid LB medium overnight, then supplemented with 50 μM CdCl 2 (b), 100 μM CdCl 2 (c), 200 μM ZnCl 2 (e) and normal LB (a, d). The cells were incubated at 37 °C for 10 h and 20 h. Web本发明涉及生物技术，具体的是一种乙酰海藻酸钠酯酶及该酯酶在降低铜绿假单胞杆菌frd1耐药性方面的应用。乙酰海藻酸钠酯酶如序列表seq id no:1中碱基所示。所述乙酰海藻酸钠酯酶在降解乙酰化海藻酸钠中的应用。所述乙酰海藻酸钠酯酶在防治铜绿假单胞杆菌感染中的应用 …

WebAug 28, 2024 · Finally, the prokaryotic expression vector pEASY®-Blunt E1-IrUGT86A1 was successfully used to express about 53 KD of IrUGT86A1-like protein. This research builds … WebMay 1, 2024 · The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. Upon reaching an OD 600 of 0.6–0.8, expression in the transformed cells was induced using 1 mmol·L −1 isopropyl β - d-1-thiogalactopyranoside (IPTG), and the proteins were collected at 8 h after induction.

WebApr 13, 2024 · 将pEASY®-Blunt E1-Brachyury导入Trans 10大肠杆菌感受态细胞中，用SOB无菌液体培养基(含100 μg/mL氨苄青霉素)于37 ℃、200 r/min条件下扩增培养1 h。取菌液涂布于LB固体培养基中，置37 ℃生化培养箱中倒置培养14 h后，挑取单克隆菌落加入SOB无菌液体培养基(含100 μg/mL氨苄 ... WebELISA. 首页 > 产品中心 > 基因表达 > pEASY 系列原核表达载体. 谁说欲速则不达？. 快速：利用DNA拓扑异构酶I，室温5分钟，快速克隆。. 简单：酶已偶联到载体上，只需加入片段即可。. 高效：载体（已偶联酶）和片段双分子碰撞，连接成功率高。. pEASY® -Blunt E1 ...

WebpEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 …

WebAug 28, 2024 · A positive colony harboring the recombinant plasmid was identified by colony PCR , confirmed by commercial sequencing and designated as pEASY-Blunt E1-ALDH. … glock discount for first respondersWebNov 19, 2016 · The pEASY-Blunt E1-trxyn10 vector was transformed into E. coli BL21 (DE3), and the positive clones were isolated for TrXyn10 expression. The transformants were cultured overnight in LB culture medium with 100 µg/ml ampicillin at 37 °C and 220 rpm. Then, 1 ml of the cells was added to 100 ml of LB medium at 25 °C and 220 rpm. glock discount programWebThe pEASY-Blunt E1-trxyn10 vector was trans-formed into E. coli BL21 (DE3), and the positive clones were isolated for TrXyn10 expression. The transformants were cultured … glock discount for military